School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
Chembiochem. 2013 Feb 11;14(3):388-94. doi: 10.1002/cbic.201200587. Epub 2013 Jan 10.
FSN1, a gene isolated from the sugar-cane pathogen Fusarium sacchari, encodes a 4707-residue nonribosomal peptide synthetase consisting of three complete adenylation, thiolation and condensation modules followed by two additional thiolation and condensation domain repeats. This structure is similar to that of ferricrocin synthetase, which makes a siderophore that is involved in intracellular iron storage in other filamentous fungi. Heterologous expression of FSN1 in Aspergillus oryzae resulted in the accumulation of a secreted metabolite that was identified as ferrirhodin. This siderophore was found to be present in both mycelium and culture filtrates of F. sacchari, whereas ferricrocin is found only in the mycelium, thus suggesting that ferricrocin is an intracellular storage siderophore in F. sacchari, whereas ferrirhodin is used for iron acquisition. To our knowledge, this is the first report to characterise a ferrirhodin synthetase gene functionally.
FSN1 是从甘蔗病原体镰刀菌中分离出来的基因，它编码一个由三个完整的腺苷酸化、硫醇化和缩合模块组成的 4707 个残基的非核糖体肽合成酶，其后是两个额外的硫醇化和缩合结构域重复。这种结构类似于铁载体合成酶，后者合成一种铁载体，参与其他丝状真菌的细胞内铁储存。FSN1 在米曲霉中的异源表达导致积累了一种分泌代谢物，被鉴定为 ferrirhodin。这种铁载体在甘蔗的菌丝体和培养滤液中都有发现，而铁载体只存在于菌丝体中，因此表明铁载体是甘蔗中的细胞内储存铁载体，而 ferrirhodin 则用于铁的获取。据我们所知，这是首次对 ferrirhodin 合成酶基因进行功能表征的报道。
