Center for Chemical Dynamics, Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA.
Chemphyschem. 2013 Feb 4;14(2):321-30. doi: 10.1002/cphc.201200962. Epub 2013 Jan 10.
Phthalocyanines have been used as photodynamic therapy (PDT) agents because of their uniquely favorable optical properties and high photostability. They have been shown to be highly successful for the treatment of cancer through efficient singlet-oxygen ((1)O(2)) production. However, due to their hydrophobic properties, the considerations of solubility and cellular location have made understanding their photophysics in vitro and in vivo difficult. Indeed, many quantitative assessments of PDT reagents are undertaken in purely organic solvents, presenting challenges for interpreting observations during practical application in vivo. With steady-state and time-resolved laser spectroscopy, we show that for axial ligated silicon phthalocyanines in aqueous media, both the water:lipophile ratio and the pH have drastic effects on their photophysics, and ultimately dictate their functionality as PDT drugs. We suggest that considering the presented photophysics for PDT drugs in aqueous solutions leads to guidelines for a next generation of even more potent PDT agents.
酞菁已被用作光动力疗法（PDT）试剂，因为它们具有独特的有利光学性质和高光稳定性。它们已被证明通过有效产生单线态氧（(1)O(2)）在癌症治疗方面非常成功。然而，由于其疏水性，对溶解度和细胞位置的考虑使得理解它们在体外和体内的光物理性质变得困难。事实上，许多 PDT 试剂的定量评估都是在纯有机溶剂中进行的，这对在体内实际应用中解释观察结果提出了挑战。通过稳态和时间分辨激光光谱，我们表明，对于轴向配位的硅酞菁在水介质中，水亲脂性比和 pH 值对其光物理性质有很大影响，并最终决定其作为 PDT 药物的功能。我们建议，考虑到在水溶液中 PDT 药物的呈现的光物理性质，为更有效的 PDT 药物的下一代提供了指导。
