Cahan C, Hoekje P L, Goldwasser E, Decker M J, Strohl K P
Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Am J Physiol. 1990 Apr;258(4 Pt 2):R1016-21. doi: 10.1152/ajpregu.1990.258.4.R1016.
We examined the relationship between the duration of hypoxic exposure and serum erythropoietin (EPO) production. Adult male Long-Evans rats were exposed to hypobaric hypoxia (HH = 0.5 atm) for a period of 15 min to 20 days. Serum for EPO by radioimmunoassay was collected immediately, 1 or 2 h after HH exposure. A significant rise in EPO levels occurred 1 h after a 30-min HH exposure that was not sustained 2 h after termination. One hour of HH exposure resulted in increased EPO levels 1 h after termination of exposure and further increased levels 2 h after termination of exposure. With prolonged exposure, serum levels remained elevated at 6 and 20 days, despite the development of polycythemia. We concluded that the hypoxic stimulus for elevation of serum EPO could be as short as 30 min and that EPO levels remained elevated after chronic HH. The experimental data were consistent with a mathematical model in which stimulated EPO production was proportional to the time of HH stimulus.
我们研究了低氧暴露持续时间与血清促红细胞生成素（EPO）产生之间的关系。成年雄性Long-Evans大鼠暴露于低压低氧环境（HH = 0.5个大气压）15分钟至20天。在HH暴露后立即、1小时或2小时收集血清，采用放射免疫分析法检测EPO。30分钟HH暴露后1小时，EPO水平显著升高，但在暴露终止2小时后未持续升高。1小时的HH暴露导致暴露终止后1小时EPO水平升高，暴露终止后2小时水平进一步升高。随着暴露时间延长，尽管出现了红细胞增多症，但血清水平在6天和20天时仍保持升高。我们得出结论，血清EPO升高的低氧刺激可能短至30分钟，并且慢性HH后EPO水平仍保持升高。实验数据与一个数学模型一致，在该模型中，刺激的EPO产生与HH刺激时间成正比。
