Hara Yurie, Watanabe Nakamichi
Department of Nutritional Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi, Tokyo, 173-8602, Japan.
Department of Health Science, Showa Women's University, 1-7-57 Taishido, Setagaya, Tokyo, 154-8533, Japan.
Heliyon. 2020 Jul 2;6(7):e04334. doi: 10.1016/j.heliyon.2020.e04334. eCollection 2020 Jul.
The aim of this study was to determine changes in gene expression associated with glucose metabolism in the liver and soleus muscles of rats exposed to hypoxia to improve work capacity under high altitude conditions. Rats were divided into normobaric normoxia (control) and normobaric hypoxia (hypoxia) groups ( = 7 each), and the hypoxia group was exposed to 10.5% oxygen for 90 min. Glucose metabolism-related gene expression was examined by real-time polymerase chain reaction. In the liver, the expression levels of the glucose utilization-related genes solute carrier family 2 member 1, glucokinase, and liver-type phosphofructokinase and the gluconeogenesis-related gene phosphoenolpyruvate carboxykinase 1 () were significantly increased upon hypoxic exposure. In contrast, gene expression in the soleus was unchanged, with the exception of . The results suggest that under hypoxia, both glucose utilization and gluconeogenesis are accelerated in the liver, and liver glycogen is degraded to maintain blood glucose level.
本研究的目的是确定暴露于低氧环境下的大鼠肝脏和比目鱼肌中与葡萄糖代谢相关的基因表达变化，以提高高原条件下的工作能力。将大鼠分为常压低氧（对照）组和常压低氧（低氧）组（每组n = 7），低氧组暴露于10.5%氧气环境90分钟。通过实时聚合酶链反应检测葡萄糖代谢相关基因的表达。在肝脏中，低氧暴露后，与葡萄糖利用相关的基因溶质载体家族2成员1、葡萄糖激酶和肝型磷酸果糖激酶以及与糖异生相关的基因磷酸烯醇式丙酮酸羧激酶1（PEPCK1）的表达水平显著增加。相比之下，比目鱼肌中的基因表达除PEPCK1外均未改变。结果表明，在低氧条件下，肝脏中的葡萄糖利用和糖异生均加速，肝糖原被降解以维持血糖水平。
