Lü Haixia, Yang Zhiqian, Lu Xiaoyun, Li Mingchuan, Jiao Qian, Chen Xinlin, Wang Yuanyuan, Zhang Yali
Institute of Neurobiology, Xi 'an Jiaotong University College of Medicine, Xi'an 710061, Shaanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2012 Oct;28(10):1216-26.
To study the attachment, proliferation and differentiation of neural stem cells (NSCs) on surface modified PHBHHx films and to establish the theory of PHBHHx application in NSCs-based brain tissue engineering. PHBHHx film was fabricated by a solution-casting method, and the morphology of the film was observed under scanning electron microscopy(SEM). The films were treated by NaOH or lipase, then the surface hydrophilic property was characterized using water contact angle measurement. NSCs were isolated from the cerebral cortex of rat embryos on embryonic day 14.5, and cultured on surface treated PHBHHx films. The morphology of NSCs attached on the film was visualized under SEM, and the survival and differentiation of NSCs were observed through immunocytochemical staining. Compared with the untreated PHBHHx films, the water contact angle of NaOH or lipase treated PHBHHx films decreased dramatically, and the number of NSCs attached significantly increased. NSCs survived well on treated PHBHHx films and differentiated into neurons and glial cells. The amelioration of hydrophilic property of PHBHHx film improved its biocompatibility with NSCs. PHBHHx can serve as a novel CNS tissue engineering biomaterial applied for NSCs transplantation, brain repairing and regeneration.
研究神经干细胞（NSCs）在表面改性聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）薄膜上的黏附、增殖和分化情况，以建立PHBHHx在基于NSCs的脑组织工程中应用的理论。通过溶液浇铸法制备PHBHHx薄膜，并在扫描电子显微镜（SEM）下观察薄膜的形态。用氢氧化钠或脂肪酶处理薄膜，然后通过测量水接触角来表征其表面亲水性。从胚胎第14.5天的大鼠胚胎大脑皮层中分离出NSCs，并在表面处理过的PHBHHx薄膜上进行培养。在SEM下观察附着在薄膜上的NSCs的形态，并通过免疫细胞化学染色观察NSCs的存活和分化情况。与未处理的PHBHHx薄膜相比，经氢氧化钠或脂肪酶处理的PHBHHx薄膜的水接触角显著降低，附着的NSCs数量明显增加。NSCs在处理过的PHBHHx薄膜上存活良好，并分化为神经元和神经胶质细胞。PHBHHx薄膜亲水性的改善提高了其与NSCs的生物相容性。PHBHHx可作为一种新型的中枢神经系统组织工程生物材料，用于NSCs移植、脑修复和再生。
