Ding Guang-Zhi, Liu Yun-Bao, Ma Shuang-Gang, Yu Shi-Shan
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(20):3083-5.
Aspergillus fumigatus, a type of endophytic fungi from Erthrophleum fordii, was fermented with GPY culture medium. Fermented liquid and mycelium were extracted from fermented products after freezing and thawing treatment. After alcohol extraction, mycelium was extracted with ethyl acetate and n-butyl alcohol, respectively. According to the results of cytotoxity of tumor cells, ethyl acetate extracts were studied for their chemical constituents. Five diketopiperazine compounds were separated and purified with silica gel, MCI and Sephadex LH-20 column chromatography, reversed-phase chromatographic column and preparative HPLC, their structures were identified as cyclo- (R-Pro-R-Phe) (1), cyclo- (trans-4-OH-D-Pro-D-Phe) (2), cyclo- (R-Tyr-S-Ile) (3), cyclo-(R-Phe-S-Ile) (4), and cyclo-(R-Val-S-Tyr) (5) by using spectral methods.
从鸡血藤中分离得到的内生真菌烟曲霉在GPY培养基中进行发酵。经过冻融处理后，从发酵产物中提取发酵液和菌丝体。菌丝体经乙醇提取后，分别用乙酸乙酯和正丁醇进行萃取。根据肿瘤细胞的细胞毒性实验结果，对乙酸乙酯提取物的化学成分进行了研究。通过硅胶柱色谱、MCI柱色谱、Sephadex LH - 20柱色谱、反相色谱柱和制备型高效液相色谱法分离并纯化出5种二酮哌嗪类化合物，采用光谱方法鉴定其结构分别为环-（R-脯氨酸-R-苯丙氨酸）（1）、环-（反式-4-羟基-D-脯氨酸-D-苯丙氨酸）（2）、环-（R-酪氨酸-S-异亮氨酸）（3）、环-（R-苯丙氨酸-S-异亮氨酸）（4）和环-（R-缬氨酸-S-酪氨酸）（5）。
