Liu Jing, Ding Guang-Zhi, Fang Lei, Yu Shi-Shan
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(20):3974-7.
The PDB culture medium was selected to ferment the endophyte strain, and the secondary metabolites of endophytic fungi Penicillium polonicum were studied. Combined application of Sephadex LH-20, ODS and HPLC chromatographies over the ethyl acetate extract of the fermented culture led to the isolation of 6 compounds. By spectral methods, the structures were elucidated as [3, 5-dihydroxy-2-(7-hydroxy-octanoyl)]-ethylphenylacetate (1), (3, 5-dihydroxy-2- octanoyl)-ethyl phenylacetate (2), (5, 7-di- hydroxy-9-heptyl)-isobenzo pyran-3-one (3), 3-(hydroxymethyl) 4-(1E)-1- propen-1-yl-(1R, 2S, 5R, 6S)-7-oxabicyclo [4.1.0] hept-3-ene-2, 5-diol (4), (E)-2-methoxy-3-(prop-1-enyl) phenol (5) and p-hydroxylphenylethanol (6).
选用马铃薯葡萄糖肉汤（PDB）培养基对内生菌株进行发酵，并对波兰青霉内生真菌的次生代谢产物进行了研究。采用葡聚糖凝胶LH - 20、十八烷基硅烷键合硅胶（ODS）和高效液相色谱（HPLC）等色谱方法对发酵培养物的乙酸乙酯提取物进行分离，得到6种化合物。通过光谱方法，确定其结构分别为[3, 5 - 二羟基 - 2 - (7 - 羟基 - 辛酰基)] - 乙基苯乙酸酯（1）、(3, 5 - 二羟基 - 2 - 辛酰基) - 乙基苯乙酸酯（2）、(5, 7 - 二羟基 - 9 - 庚基) - 异苯并吡喃 - 3 - 酮（3）、3 - (羟甲基) 4 - (1E) - 1 - 丙烯 - 1 - 基 - (1R, 2S, 5R, 6S) - 7 - 氧杂双环[4.1.0]庚 - 3 - 烯 - 2, 5 - 二醇（4）、(E) - 2 - 甲氧基 - 3 - (丙 - 1 - 烯基)苯酚（5）和对羟基苯乙醇（6）。
