[丹吉尔青霉内生真菌次生代谢产物的研究]

[Study on secondary metabolites of endophytic fungi Penicillium dangeardii].

作者信息

Lv Hai-ning, Ding Guang-zhi, Liu Yun-bao, Qu Jing

出版信息

Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1759-61.

Abstract

Endophytic fungi Penicillium dangeardii, isolated from Lysidice rhodostegia Hance root, was fermented and the secondary metabolites were studied. By means of Sephadex LH-20 column chromatography, ODS column chromatography and PHPLC over the fermented culture, 5 compounds were isolated. By using ESI-MS and NMR, the structures of the compounds were determined as N-[9-(β- D-ribofuranosyl)-9H-purin-6-yl]-L-aspartic acid (1), 3-caffeoylquinic acid (2), 4-caffeoylquinic acid (3), and 5-caffeoylquinic acid (4), 3-hydroxy-benzoic acid-4-O-β-D-glucopyranoside (5).

摘要

从红榄李根部分离得到内生真菌丹吉尔青霉，并对其进行发酵，研究其次级代谢产物。通过对发酵培养物进行葡聚糖凝胶LH - 20柱色谱、反相硅胶柱色谱和制备型高效液相色谱，分离得到5种化合物。利用电喷雾电离质谱和核磁共振确定这些化合物的结构分别为N - [9 - (β - D - 呋喃核糖基)-9H - 嘌呤 - 6 - 基]-L - 天冬氨酸(1)、3 - 咖啡酰奎宁酸(2)、4 - 咖啡酰奎宁酸(3)、5 - 咖啡酰奎宁酸(4)、3 - 羟基苯甲酸 - 4 - O - β - D - 吡喃葡萄糖苷(5)。