UR02SP13 Research Unit, Ministry of Public Health, Tunisia, Tunisia.
J Appl Microbiol. 2013 Jun;114(6):1559-74. doi: 10.1111/jam.12132. Epub 2013 Feb 1.
Candida albicans is one of the most medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzyme electrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) and DNA-based methods called exact typing methods because they generate unambiguous and highly reproducible typing data (including microsatellite length polymorphism and multi-locus sequence typing). In this review, the main molecular methods used for C. albicans strain typing are summarized, and their advantages and limitations are discussed with regard to their discriminatory power, reproducibility, cost and ease of performance.
白色念珠菌是最重要的医学真菌之一，因为它既是一种高频率的共生微生物，也是一种导致浅表和侵袭性感染的病原微生物。对于理解其生物学、流行病学和种群结构而言，菌株分型和物种划定至关重要。为此，已经使用了广泛的分子技术，包括非 DNA 方法（多位点酶电泳）、常规 DNA 方法（电泳核型分析、随机扩增多态性 DNA、扩增片段长度多态性、酶切分析伴或不伴杂交、重复序列 PCR）和被称为准确分型方法的 DNA 方法，因为它们产生明确且高度可重复的分型数据（包括微卫星长度多态性和多位点序列分型）。在这篇综述中，总结了用于白色念珠菌菌株分型的主要分子方法，并就其分辨能力、可重复性、成本和操作简便性讨论了它们的优缺点。
