Center for Food Safety and Applied Nutrition, Office of Regulatory Science, United States Food and Drug Administration-U.S. FDA, College Park, Maryland 20740, USA.
J Agric Food Chem. 2013 Feb 6;61(5):1060-6. doi: 10.1021/jf304223a. Epub 2013 Jan 23.
Recent work has shown that the presence of the species Pinus armandii , even when occurring as species mixtures of pine nuts, is correlated with taste disturbance (dysgeusia), also referred to as "pine mouth". Because of this known possibility of pine nut mixtures, a need was identified for a rapid streamlined assay to detect the presence of this species in the presence of other types of pine nuts. A locked nucleic acid probe was employed in a real-time polymerase chain reaction (RT-PCR) format to detect a single nucleotide polymorphism (SNP) unique to this species. This assay was able to detect P. armandii in homogenates down to ∼1% concentration (the lowest level tested) in the presence of several commonly co-occurring and closely related species of pine and should prove to be a useful tool for the detection of this species in food products.
最近的研究表明，即使作为松子的物种混合物存在，松属华山松的存在也与味觉障碍（味觉障碍）有关，也称为“松口”。由于这种已知的松仁混合物的可能性，需要一种快速简化的测定法来检测其他类型的松子中该物种的存在。在实时聚合酶链反应（RT-PCR）格式中使用锁核酸探针来检测该物种特有的单个核苷酸多态性（SNP）。该测定法能够在存在几种常见共存和密切相关的松树物种的情况下，从匀浆中检测到低至约 1%浓度（测试的最低水平）的华山松，并且应该被证明是在食品中检测该物种的有用工具。
