Department of Chemistry, Institute of Chemistry, Technology, and Metallurgy, University of Belgrade, Belgrade, Serbia.
Nutrition. 2013 Feb;29(2):431-5. doi: 10.1016/j.nut.2012.07.018.
Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties.
The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas.
In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals.
Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.
与婴儿配方奶粉相比，早期和纯母乳喂养对婴儿有显著的健康益处。本研究旨在通过研究母乳和标准婴儿配方奶粉对非血管平滑肌收缩的影响及其抗氧化特性来比较它们。
使用大鼠子宫平滑肌收缩模型系统来检查母乳和配方奶的药理作用。电子顺磁共振自旋捕获光谱法用于比较在第九周哺乳期获得的母乳与商业婴儿配方奶粉在 Fenton 反应中对羟自由基生成的抗氧化能力。在母乳和婴儿配方奶粉中测定超氧化物歧化酶、谷胱甘肽过氧化物酶和巯基的活性。
与婴儿配方奶粉相比，母乳对分离的非血管平滑肌有舒张作用。一般来说，母乳的抗氧化活性高于婴儿配方奶粉。在所有样本中，羟基自由基的产生导致碳中心和抗坏血酸自由基的形成。
由于存在特定的酶成分，如人超氧化物歧化酶，人乳对非血管平滑肌具有直接的药理舒张作用，并提供更好的抗氧化保护。我们提出，这些作用通过支持平滑肌的正常工作和维持氧化还原平衡，对婴儿的胃肠道有益，这可能是母乳喂养有益于健康的机制之一。
