Department of Psychiatry and Behavioral Sciences, School of Medicine, Johns Hopkins University, Ross 615, 720 Rutland Ave, Baltimore, MD 21205, USA.
Physiol Behav. 2013 Feb 17;110-111:109-14. doi: 10.1016/j.physbeh.2013.01.003. Epub 2013 Jan 10.
Evidence in the literature raises the possibility that alterations in neuropeptide Y (NPY) in the dorsomedial hypothalamus (DMH) may contribute to hyperphagia leading to body weight gain. Previously, we have shown that compared to AAVGFP controls, adeno-associated virus (AAV)-mediated overexpression of NPY in the DMH of lean rats resulted in significantly higher body weight gain that was attributed to increased food intake, and this was further exacerbated by a high-fat diet. Here, we tested AAVNPY and AAVGFP control rats in a brief-access taste procedure (10-s trials, 30-min sessions) to an array of sucrose concentrations under ad libitum and partial food and water access conditions. The test allows for some segregation of the behavioral components by providing a measure of trial initiation (appetitive) and unconditioned licks at each concentration (consummatory). Consistent with previous findings suggesting that NPY has a primary effect on appetitive function, overexpression of DMH NPY did not significantly alter concentration-dependent licking response to sucrose but when tested in a non-restricted food and water schedule, AAVNPY rats initiated significantly more sucrose trials compared to AAVGFP controls in a brief-access taste test.
文献中的证据表明，背内侧下丘脑（DMH）中神经肽 Y（NPY）的改变可能导致摄食量增加，从而导致体重增加。此前，我们已经表明，与 AAVGFP 对照组相比，瘦鼠 DMH 中 NPY 的腺相关病毒（AAV）过表达导致体重显著增加，这归因于食物摄入量增加，而高脂肪饮食进一步加剧了这种情况。在这里，我们在自由摄取和部分食物和水摄取条件下，通过短暂接入味觉程序（10 秒试验，30 分钟试验），对 AAVNPY 和 AAVGFP 对照组大鼠进行了一系列蔗糖浓度测试。该测试通过提供每个浓度的试验启动（食欲）和非条件舔舐的测量值，允许对行为成分进行一定程度的分离（摄取）。与先前的研究结果一致，表明 NPY 对食欲功能有主要影响，DMH NPY 的过表达并没有显著改变蔗糖的浓度依赖性舔舐反应，但在非限制食物和水时间表中进行测试时，与 AAVGFP 对照组相比，AAVNPY 大鼠在短暂接入味觉测试中启动了明显更多的蔗糖试验。
