Schaffar B P, Wolfbeis O S
Department of Enzyme Technology, GBF, Braunschweig, FRG.
Biosens Bioelectron. 1990;5(2):137-48. doi: 10.1016/0956-5663(90)80004-w.
A fast responding glucose biosensor for the continuous determination of glucose is presented. The biosensor is based on an oxygen optrode, which measures the consumption of oxygen via dynamic quenching of the fluorescence of an indicator by molecular oxygen. Glucose oxidase (GOD) is immobilised onto the surface of this oxygen optrode by adsorption to carbon black and by crosslinking with glutardialdehyde. Carbon black is used as an optical isolation to protect the optrode from the interference of ambient light and sample fluorescence. The measurements were performed in a flow through cell with air saturated glucose standard solutions (phosphate buffered saline pH 6.9). The effect of four different qualities of GOD in relation to response times (the time required to reach 90% of the steady-state signal, tau 90, was 8-60 s, the linear analytical range (0.01 to 2 mM glucose) and the long-term stability (tau 1/2 was 1-20 weeks) were investigated. A simple device is presented capable of enlarging the analytical range up to 200 mM glucose concentration.
介绍了一种用于连续测定葡萄糖的快速响应葡萄糖生物传感器。该生物传感器基于氧电极，通过分子氧对指示剂荧光的动态猝灭来测量氧的消耗。葡萄糖氧化酶（GOD）通过吸附到炭黑上并与戊二醛交联固定在该氧电极的表面。炭黑用作光学隔离材料，以保护电极免受环境光和样品荧光的干扰。测量在流通池中进行，使用空气饱和的葡萄糖标准溶液（磷酸盐缓冲盐水，pH 6.9）。研究了四种不同质量的GOD对响应时间（达到稳态信号的90%所需的时间，τ90为8 - 60秒）、线性分析范围（0.01至2 mM葡萄糖）和长期稳定性（半衰期τ1/2为1 - 20周）的影响。还介绍了一种简单的装置，能够将分析范围扩大到高达200 mM的葡萄糖浓度。
