National Institute of Advanced Industrial, Science and Technology (AIST), Midorigaoka, Ikeda 563-8577, Japan.
Talanta. 2010 Nov 15;83(1):61-5. doi: 10.1016/j.talanta.2010.08.039.
Optical biosensor arrays for rapidly determining the glucose concentrations in a large number of beverage and blood samples were developed by immobilizing glucose oxidase (GOD) on oxygen sensor layer. Glucose oxidase was first encapsulated in silica based gels through sol-gel approach and then immobilized on 96-well microarrays integrated with oxygen sensing film at the bottom. The oxygen sensing film was made of an organically modified silica film (ORMOSIL) doped with tris(4,7-diphenyl-1,10-phenanthroline) ruthenium dichloride (Ru(dpp)(3)Cl(2)). The oxidation reaction of glucose by glucose oxidase could be monitored through fluorescence intensity enhancement due to the oxygen consumption in the reaction. The luminescence changing rate evaluated by the dynamic transient method (DTM) was correlated with the glucose concentration with the wide linear range from 0.1 to 5.0mM (Y=13.28X-0.128, R=0.9968) and low detection limit (0.06 mM). The effects of pH and coexisting ions were systemically studied. The results showed that the optical biosensor arrays worked under a wide range of pH value, and normal interfering species such as Na(+), K(+), Cl(-), PO(4)(3-), and ascorbic acid did not cause apparent interference on the measurement. The activity of glucose oxidase was mostly retained even after 2-month storage, indicating their long-term stability.
通过将葡萄糖氧化酶（GOD）固定在氧传感器层上，开发了用于快速测定大量饮料和血液样本中葡萄糖浓度的光学生物传感器阵列。首先通过溶胶-凝胶法将葡萄糖氧化酶封装在基于硅的凝胶中，然后将其固定在带有氧敏膜的 96 孔微阵列上。氧敏膜由有机改性硅膜（ORMOSIL）掺杂三（4,7-二苯基-1,10-菲咯啉）钌二氯化物（Ru（dpp）（3）Cl 2）制成。葡萄糖氧化酶对葡萄糖的氧化反应可以通过反应中氧气消耗引起的荧光强度增强来监测。通过动态瞬态法（DTM）评估的发光变化率与葡萄糖浓度具有宽线性范围（0.1 至 5.0mM，Y=13.28X-0.128，R=0.9968）和低检测限（0.06mM）相关。系统研究了 pH 值和共存离子的影响。结果表明，光学生物传感器阵列在宽 pH 值范围内工作，并且正常的干扰物质如 Na(+)、K(+)、Cl(-)、PO(4)(3-)和抗坏血酸不会对测量产生明显干扰。即使在 2 个月的储存后，葡萄糖氧化酶的活性也大多保留，表明其具有长期稳定性。
