Department of Biochemistry and Cell Biology, Rice University, Houston, TX, USA.
Bioresour Technol. 2013 Feb;130:398-405. doi: 10.1016/j.biortech.2012.10.154. Epub 2012 Nov 7.
Escherichia coli strains HL2765 and HL27659k harboring pRU600 and pKK313 were examined for succinate production under aerobic conditions using galactose, sucrose, raffinose, stachyose, and mixtures of these sugars extracted from soybean meal and soy solubles. HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 87mM and 98mM hexose of soybean meal extract and produced 83mM and 95mM succinate, respectively. While using soy solubles extract, HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 160mM and 187mM hexose and produced 158mM and 183mM succinate, respectively. Succinate yield of HL2765(pKK313)(pRU600) was low as compared to that of HL27659k(pKK313)(pRU600) while using acid hydrolysate of soybean meal or soy solubles extracts. Maximum succinate production of 312mM with a molar yield of 0.82mol/mol hexose was obtained using soy solubles hydrolysate by HL27659k(pKK313)(pRU600). This study demonstrated the use of soluble carbohydrates of the renewable feedstock, soybean as an inexpensive carbon source to produce succinate by fermentation.
含有 pRU600 和 pKK313 的大肠杆菌 HL2765 和 HL27659k 在有氧条件下使用半乳糖、蔗糖、棉子糖、水苏糖以及从豆粕和大豆可溶物中提取的这些糖的混合物作为碳源进行了琥珀酸生产的研究。HL2765(pKK313)(pRU600) 和 HL27659k(pKK313)(pRU600) 消耗了 87mM 和 98mM 来自豆粕提取物的己糖，并分别产生了 83mM 和 95mM 琥珀酸。而使用大豆可溶物提取物时，HL2765(pKK313)(pRU600) 和 HL27659k(pKK313)(pRU600) 消耗了 160mM 和 187mM 己糖，并分别产生了 158mM 和 183mM 琥珀酸。与 HL27659k(pKK313)(pRU600) 相比，HL2765(pKK313)(pRU600) 在使用豆粕或大豆可溶物酸水解物作为碳源时，琥珀酸的产率较低。HL27659k(pKK313)(pRU600) 使用大豆可溶物水解物可获得 312mM 的最大琥珀酸产量，摩尔产率为 0.82mol/mol 己糖。本研究表明，可利用可再生原料大豆的可溶性碳水化合物作为发酵生产琥珀酸的廉价碳源。
