Advanced Water Management Centre, The University of Queensland, Brisbane, Queensland 4072, Australia.
Bioresour Technol. 2013 Feb;129:599-605. doi: 10.1016/j.biortech.2012.11.098. Epub 2012 Dec 4.
Bioelectrochemical systems (BESs) are emerging as a technology with diverse future applications. Anode-associated microbial diversity and activity are known to change over time, but the consequences of these dynamics on BES functioning are poorly understood. A novel BES with exchangeable anodic electrodes that facilitates characterisation of microbial communities over time was constructed. The BES, received a mixture of volatile fatty acids and produced 0.13 mA cm(-2) of anodic electrode surface, leading to the removal of 14 g chemical oxygen demand per m2 electrode per day at a coulombic efficiency of 76%. Pyrosequencing of 16S rRNA genes revealed no differences in the diversity of microbial communities associated with different electrodes within a single time point. This finding validates the design for temporal studies as changes in microbial diversity observed over time can be related to community development rather than spatial variation within the reactor.
生物电化学系统（BES）作为一种具有多种未来应用的技术正在兴起。已知阳极相关微生物的多样性和活性随时间而变化，但这些动态对 BES 功能的影响还知之甚少。构建了一种具有可交换阳极电极的新型 BES，可方便地随时间对微生物群落进行特征描述。该 BES 接收混合挥发性脂肪酸，并在阳极电极表面产生 0.13 mA cm(-2) 的电流，每天每平方米电极去除 14 g 化学需氧量，库仑效率为 76%。16S rRNA 基因的焦磷酸测序显示，在单一时间点，与不同电极相关的微生物群落的多样性没有差异。这一发现验证了该设计用于时间研究的合理性，因为随着时间的推移观察到的微生物多样性变化可以与群落发展相关，而不是与反应器内的空间变化相关。
