Department of Food, Agricultural and Biological Engineering, Ohio State University, 590 Woody Hayes Drive, Columbus, OH 43210, USA.
Bioresour Technol. 2013 Feb;129:281-8. doi: 10.1016/j.biortech.2012.10.137. Epub 2012 Nov 9.
The objective of this work was to evaluate methanogenesis in relation to the changes in performance and microbial diversity of cellulose-fed microbial fuel cells (MFCs). Replicate MFCs were inoculated with a ruminal microbial consortium and operated under 20 (R20Ω) or 100 Ω (R100Ω) external resistances. During the first week of operation, 0.31 and 0.44 mmol l(-1) of methane were produced in the R20Ω and R100Ω MFCs, respectively. Methanogenesis was, however, suppressed to undetectable levels within 90 days of operation, accompanied with increased current production and improved coulombic efficiency. Suppressed methanogenesis coincided with changes in the concentrations of short chain fatty acids and a decrease in the microbial diversity. The results demonstrated that methanogenesis was active during the early stage of cellulose-fed MFCs but this activity declined over prolonged operation.
本研究旨在评估产甲烷菌与纤维素-fed 微生物燃料电池（MFC）性能变化和微生物多样性之间的关系。采用瘤胃微生物混合菌群接种重复 MFC，分别在 20（R20Ω）或 100 Ω（R100Ω）外电阻下运行。在运行的第一周，R20Ω 和 R100Ω MFC 分别产生了 0.31 和 0.44 mmol l(-1)的甲烷。然而，产甲烷菌在运行 90 天内被抑制到无法检测的水平，同时伴随着电流产生的增加和库仑效率的提高。抑制产甲烷菌与短链脂肪酸浓度的变化和微生物多样性的降低同时发生。结果表明，在纤维素-fed MFC 的早期阶段产甲烷菌是活跃的，但这种活性在长时间运行后会下降。
