Graduate School of East-West Medical Science, Kyung Hee University, Gyeonggi-do 449-701, Republic of Korea.
Anal Biochem. 2013 Apr 1;435(1):68-73. doi: 10.1016/j.ab.2012.12.018. Epub 2013 Jan 8.
A water-soluble, dual reactive hydrazide/click crosslinker (ethynyl hydrazide, EH) was synthesized and characterized. A model antibody, human immunoglobulin G (hIgG), was ethynylated by conventional oxidation/hydrazide reactions with the hydrazide moiety of EH. The terminal alkyne conjugated to the glycan of hIgG was easily functionalized by quantitative and bioorthogonal Cu(I)-catalyzed azide-alkyne cycloaddition. The potential of the hydrazide/click crosslinker as a reagent to functionalize antibodies was demonstrated with fluorophore labeling and antibody immobilization.
一种水溶性的、双反应性的酰腙/点击交联剂（乙炔基酰腙，EH）被合成并进行了表征。通过传统的氧化/酰腙反应，将酰腙部分的 EH 用于人免疫球蛋白 G（hIgG）的乙醯化。末端炔基与 hIgG 的聚糖通过定量和生物正交的 Cu（I）催化的叠氮-炔环加成反应很容易进行功能化。酰腙/点击交联剂作为一种用于抗体功能化的试剂的潜力，通过荧光标记和抗体固定化进行了证明。
