Augusco Marco Aurélio Carmona, Sarri Daniela Abram, Panontin Juliane Farinelli, Rodrigues Maria Angélica Melo, Fernandes Rachel de Moura Nunes, Silva Juliana Fonseca Moreira da, Cardoso Claudia Andrea Lima, Rambo Magale Karine Diel, Scapin Elisandra
Postgraduate Program in Environmental Sciences-CIAMB, Federal University of Tocantins, Campus of Palmas, Palmas 77001-090, TO, Brazil.
Environmental Engineering Course, Chemistry Laboratory, Block II, Federal University of Tocantins, Campus of Palmas, Palmas 77001-090, TO, Brazil.
Plants (Basel). 2023 Jun 15;12(12):2327. doi: 10.3390/plants12122327.
The study of phytotherapy in dentistry holds great relevance because of the scarcity of research conducted on the treatment of oral pathologies, specifically, caries and periodontal disease. Therefore, this research aimed to analyze the chemical composition of extracts from Aubl. leaves, evaluate their toxicity, and assess their antioxidant and antimicrobial properties against , , and . Three extracts were prepared using assisted ultrasound and the Soxhlet apparatus, namely, Crude Ultrasound Extract (CUE), Crude Soxhlet Extract (CSE), and the Ethanol Soxhlet Extract (ESE). Flavonoids, tannins, and saponins were detected in the chemical analysis, while LC-DAD analysis revealed the presence of caffeic acid, sinapic acid, rutin, quercetin, luteolin, kaempferol, and apigenin in all extracts. GC-MS analysis identified stigmasterol and β-sitosterol in the CUE and CSE. The ESE showed higher antioxidant activity (2.98 ± 0.96 and 4.93 ± 0.90) determined by the DPPH• and ABTS• methods, respectively. In the toxicity evaluation, the CUE at 50 μg/mL and the ESE at 50 μg/mL stimulated the growth of roots, while all extracts inhibited root growth at 750 μg/mL. None of the extracts exhibited toxicity against . Antibacterial activity was observed in all extracts, particularly against the microorganisms and . However, no antifungal activity against was detected. These results suggest that extracts of have therapeutic potential for controlling microorganisms in the oral microbiota.
由于针对口腔疾病（特别是龋齿和牙周病）治疗的研究稀缺，植物疗法在牙科领域的研究具有重大意义。因此，本研究旨在分析Aubl.叶提取物的化学成分，评估其毒性，并评估其对、和的抗氧化和抗菌性能。使用辅助超声和索氏提取器制备了三种提取物，即粗超声提取物（CUE）、粗索氏提取物（CSE）和乙醇索氏提取物（ESE）。化学分析检测到了黄酮类化合物、单宁和皂苷，而LC-DAD分析表明所有提取物中均存在咖啡酸、芥子酸、芦丁、槲皮素、木犀草素、山奈酚和芹菜素。GC-MS分析在CUE和CSE中鉴定出豆甾醇和β-谷甾醇。通过DPPH•和ABTS•方法测定，ESE分别显示出较高的抗氧化活性（2.98±0.96和4.93±0.90）。在毒性评估中，50μg/mL的CUE和50μg/mL的ESE刺激了根的生长，而所有提取物在750μg/mL时均抑制根的生长。没有一种提取物对表现出毒性。在所有提取物中均观察到抗菌活性，尤其是对微生物和。然而，未检测到对的抗真菌活性。这些结果表明，Aubl.叶提取物在控制口腔微生物群中的微生物方面具有治疗潜力。
