Sugie H, Tsurui S, Ishikawa A, Matsuda F, Sugie Y, Igarashi Y, Fujise Y
Department of Pediatrics, Hamamatsu University Medical School.
No To Hattatsu. 1990 Mar;22(2):166-72.
The brain metabolites were studied in the neonatal hypothyroid rat brain using 31P and 1H nuclear magnetic resonance (NMR) spectroscopy. In vivo 31P spectroscopy of the brains in hypothyroid rats showed the tendency of decrease in phosphocreatine/Pi and phosphocreatine/ATP ratio, suggesting suppressed brain energy status. On proton analysis using perchloric acid extracts of the brain, 10 metabolites were easily recognized. Quantitative analysis of individual metabolites revealed decreased contents of GABA, NAA (N-acetyl aspartate) and phosphocreatine and increased contents of taurine in hypothyroid rat. It was concluded that NMR is a useful and promising tool to evaluate brain metabolites.
利用31P和1H核磁共振（NMR）光谱技术对新生甲状腺功能减退大鼠脑内的代谢物进行了研究。甲状腺功能减退大鼠脑内的活体31P光谱显示磷酸肌酸/无机磷酸盐和磷酸肌酸/三磷酸腺苷比值有降低趋势，提示脑能量状态受到抑制。对脑高氯酸提取物进行质子分析时，可轻松识别出10种代谢物。对单个代谢物的定量分析显示，甲状腺功能减退大鼠脑内γ-氨基丁酸、N-乙酰天门冬氨酸（NAA）和磷酸肌酸含量降低，牛磺酸含量增加。得出的结论是，核磁共振是评估脑代谢物的一种有用且有前景的工具。
