Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha Street 12/16, 90-237, Lodz, Poland.
Environ Sci Pollut Res Int. 2018 Jan;25(3):2738-2743. doi: 10.1007/s11356-017-0571-4. Epub 2017 Nov 14.
The chemical 2,4-dichlorophenoxyacetic acid (2,4-D) is used in agriculture as a herbicide. Its intensive use has an adverse effect on the environment. This study involved examining the degradation of 2,4-D compound by the filamentous fungus Umbelopsis isabellina. After 5 days of incubation, 98% of the herbicide (added at 25 mg L) was found to be removed. The elimination of 2,4-D by U. isabellina was connected with the formation of 2,4-dichlorophenol (2,4-DCP), which resulted in a 60% decrease in the sample toxicity toward Artemia franciscana larvae. The metabolism of 2,4-D was inhibited by the addition of metyrapone, a known cytochrome P450 inhibitor. It provides evidence that cytochrome P450 system is involved in 2,4-D metabolism in U. isabellina.
化学物质 2,4-二氯苯氧乙酸（2,4-D）在农业中用作除草剂。其大量使用对环境有不利影响。本研究涉及丝状真菌 Umbellopsis isabellina 对 2,4-D 化合物的降解。孵育 5 天后，发现 98%的除草剂（添加量为 25mg/L）被去除。U. isabellina 对 2,4-D 的消除与 2,4-二氯苯酚（2,4-DCP）的形成有关，这导致对 Artemia franciscana 幼虫的样品毒性降低了 60%。添加米曲酮（一种已知的细胞色素 P450 抑制剂）抑制了 2,4-D 的代谢。这为细胞色素 P450 系统参与 U. isabellina 中 2,4-D 代谢提供了证据。
