School of Dentistry, Research Wing, University of Liverpool, Liverpool, United Kingdom.
J Dent Educ. 2013 Jan;77(1):68-71.
Formalin is a known carcinogen, so there is a need to establish whether a safer alternative is available for the sterilization of human teeth destined for use in clinical training. Any disinfectant that is not capable of sterilizing 100 percent of the samples tested should be considered a failure. In this study, biofilms of oral bacteria were grown on previously autoclaved extracted human teeth. These biofilm-laden teeth were then screened against a range of disinfectants for an exposure time of seven days in a laboratory refrigerator. Culture methods were employed to validate the sterility of the tooth samples. Five percent Virkon and Gigasept PA proved effective against the laboratory model of disinfection and were carried forward to challenge freshly extracted human teeth. Gigasept PA was the only disinfectant that sterilized 100 percent of the tooth samples. Gigasept PA should be considered a safer and effective alternative to formalin for the sterilization of extracted teeth destined for teaching purposes.
甲醛是一种已知的致癌物质，因此需要确定是否有更安全的替代品可用于消毒用于临床培训的人类牙齿。任何不能对 100%测试样本进行消毒的消毒剂都应被视为失败。在这项研究中，口腔细菌的生物膜被种植在先前经过高压灭菌的提取的人类牙齿上。然后，这些载有生物膜的牙齿在实验室冰箱中暴露于各种消毒剂中七天，以进行筛选。采用培养方法验证牙齿样本的无菌性。5%的 Virkon 和 Gigasept PA 对实验室模型的消毒有效，并被进一步用于挑战新鲜提取的人类牙齿。Gigasept PA 是唯一能够对 100%的牙齿样本进行消毒的消毒剂。Gigasept PA 应被视为甲醛的更安全、更有效的替代品，用于消毒用于教学目的的提取牙齿。
