Tijare Manisha, Smitha D, Kasetty Sowmya, Kallianpur Shreenivas, Gupta Sandeep, Amith Hv
Department of Oral Pathology and Microbiology, People's College of Dental Sciences and Research Centre, People's University, Bhopal, Madhya Pradesh, India.
Department of Public Health Dentistry, People's College of Dental Sciences and Research Centre, People's University, Bhopal, Madhya Pradesh, India.
J Oral Maxillofac Pathol. 2014 Jan;18(1):14-8. doi: 10.4103/0973-029X.131883.
Extracted human teeth are routinely used in dentistry to learn technical and preclinical skills. Centers for Disease Control and Prevention (CDC) has adopted guidelines for infection control of extracted teeth used for research and teaching, requiring that teeth be sterilized before use. Many of the proposed disinfection methods starting from use of formalin, sodium hypochlorite and to autoclaving have their own drawbacks and may not be practical.
To assess the effectiveness of vinegar for disinfection/sterilization of extracted human teeth.
A total of 80 extracted non-carious human teeth were kept in seven disinfectant media-10% formalin, 3% hydrogen peroxide, 5.25% sodium hypochlorite, 70% alcohol, vinegar, neem extract and normal saline. Ten samples were placed in each disinfectant individually for a period of 7 days, at room temperature. In all, 10 teeth were treated with microwave irradiation at 650 W for 3 min. Later, teeth from each group were placed individually in separate test tubes containing 10 ml of tryptic soy broth at 37°C for 48 h to observe the evidence of growth of microorganisms. Semiquantitative analysis of all the samples was done in Clade agar at 37°C for 48 h.
The number of teeth disinfected in each group was compared using Chi square test.
10% formalin, 3% hydrogen peroxide and vinegar were totally effective. The result was statistically significant with a Chi square value of 61.414 and P < 0.001.
Vinegar can be used as an effective disinfectant medium for extracted human teeth.
在牙科领域，常使用拔除的人类牙齿来学习技术和临床前技能。美国疾病控制与预防中心（CDC）已采用针对用于研究和教学的拔除牙齿的感染控制指南，要求牙齿在使用前进行消毒。许多提议的消毒方法，从使用福尔马林、次氯酸钠到高压灭菌，都有各自的缺点，可能并不实用。
评估醋对拔除的人类牙齿进行消毒/灭菌的效果。
总共80颗拔除的无龋人类牙齿保存在七种消毒介质中——10%福尔马林、3%过氧化氢、5.25%次氯酸钠、70%酒精、醋、印楝提取物和生理盐水。每组分别放置10个样本，在室温下放置7天。总共10颗牙齿用650瓦的微波辐射处理3分钟。之后，将每组的牙齿分别置于装有10毫升胰蛋白胨大豆肉汤的单独试管中，在37°C下放置48小时，以观察微生物生长的迹象。所有样本在37°C的克氏琼脂中进行48小时的半定量分析。
使用卡方检验比较每组消毒牙齿的数量。
10%福尔马林、3%过氧化氢和醋完全有效。卡方值为61.414，P < 0.001，结果具有统计学意义。
醋可作为拔除的人类牙齿的有效消毒介质。
