Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Anal Bioanal Chem. 2013 May;405(13):4477-85. doi: 10.1007/s00216-012-6668-1. Epub 2013 Jan 15.
Angelica sinensis (Oliv.) Diels ("Danggui" in Chinese) is one of the most commonly used traditional Chinese medicines. It has been used to invigorate blood circulation for the treatment of anemia, hypertension, chronic bronchitis, asthma, rheumatism, and cardiovascular diseases. There are a number of A. sinensis-derived dietary supplements in the US markets. However, no study have been conducted to investigate the quality of these dietary supplements. In this paper, high-performance liquid chromatographic and flow-injection mass spectrometric fingerprints were both evaluated to assess the consistency of A. sinensis-derived dietary supplements. Similarity analysis was carried out on the high-performance liquid chromatographic (HPLC) fingerprints. Meanwhile, principal component analysis (PCA) was performed on the data obtained from flow-injection mass spectrometric (FIMS) fingerprints, which can analyze each sample in 2 min, compared with 30 min required for the chromatographic fingerprint. Both methods show significant chemical differences between samples that may be due to differences in growing locations, growing conditions, harvesting times, and/or botanical processing. The loading plots obtained from PCA singled out the discriminatory ions that were responsible for chemical differences of A. sinensis-derived dietary supplements.
当归（Angelica sinensis（Oliv.）Diels）是最常用的中药之一。它被用于促进血液循环，治疗贫血、高血压、慢性支气管炎、哮喘、风湿和心血管疾病。美国市场上有许多以当归为原料的膳食补充剂。然而，目前还没有研究来调查这些膳食补充剂的质量。在本文中，我们分别使用高效液相色谱和流动注射质谱指纹图谱来评估当归衍生膳食补充剂的一致性。对高效液相色谱（HPLC）指纹图谱进行了相似性分析。同时，对流动注射质谱（FIMS）指纹图谱得到的数据进行了主成分分析（PCA），该方法可以在 2 分钟内分析每个样品，而色谱指纹图谱则需要 30 分钟。这两种方法都显示出样品之间存在显著的化学差异，这可能是由于生长地点、生长条件、收获时间和/或植物处理的不同造成的。PCA 得到的加载图确定了区分当归衍生膳食补充剂的化学差异的特征离子。
