Federal State Budgetary Institution, Research Institute of Pharmacology under the Siberian Branch of Russian Academy of Medical Sciences, Tomsk, Russia.
Stem Cell Rev Rep. 2013 Apr;9(2):140-7. doi: 10.1007/s12015-012-9423-2.
The effects of nanotechnology (electron-beam) -PEGylated (or immobilized; Im) hyaluronidase (HD) on the state of the pool of bone marrow progenitor cells and their mobilization induced by granulocyte colony stimulating factor (G-CSF) were studied. A high specific activity of the drug Im-HD on progenitor cells of different classes was demonstrated using parenteral and enteral administration. An increase in the content of erythroid (E), granulomonocytic (GM), fibroblast (F) colony-forming units (CFU) and mesenchymal stem cells (MSC) in bone marrow was shown, as well as G-CSF-induced stimulation of mobilization of precursors into the peripheral blood under the influence of Im-HD. The detected activity of this novel drug on progenitor cells indicates the potential for a safe and highly effective treatment for hematology practice and regenerative medicine.
研究了纳米技术（电子束）-聚乙二醇化（或固定化；Im）透明质酸酶（HD）对骨髓祖细胞库状态及其对粒细胞集落刺激因子（G-CSF）诱导的动员的影响。研究表明，使用肠外和肠内给药，该药物 Im-HD 对不同类别的祖细胞具有高比活性。研究表明，骨髓中红系（E）、粒细胞-单核细胞（GM）、成纤维细胞（F）集落形成单位（CFU）和间充质干细胞（MSC）的含量增加，并且在 Im-HD 的影响下，G-CSF 诱导的祖细胞向外周血的动员增加。这种新型药物对祖细胞的检测活性表明，它具有用于血液学实践和再生医学的安全、高效治疗的潜力。
