Samli H, Demir B Ç, Ozgöz A, Atalay M A, Uncu G
Department of Genetics, Faculty of Veterinary Medicine, UludaÄŸ University, Bursa, Turkey.
Genet Mol Res. 2012 Dec 21;11(4):4739-45. doi: 10.4238/2012.December.17.6.
Vascular endothelial growth factor (VEGF) regulates endothelial cell proliferation, migration and differentiation. VEGF plays a critical role in angiogenesis during placenta formation. We investigated whether VEGF gene polymorphisms are associated with recurrent pregnancy loss. Thirty-eight women with recurrent pregnancy loss and 30 control women with live-born children were recruited from 2010 to 2011 in the region of Bursa, Turkey. VEGF gene polymorphisms were assessed with PCR-RFLP analysis of DNA samples obtained from leukocytes. DNA fragments were investigated by using appropriate primers. SNP scanning was performed using MnII, BgIII, BshI2361, Hsp92II restriction enzymes for 1154 G/A, 2578 C/A, 460 C/T, and 936 C/T polymorphisms, respectively. The frequencies of 2578 C/A, 460 C/T, 936 C/T polymorphisms were not significantly different between the controls and women with recurrent pregnancy loss. However, the prevalence of the 1154 G/A polymorphism A/A genotype was significantly higher in the recurrent pregnancy loss group (23.7 vs 3.4%). One of the four common polymorphisms of the VEGF gene was found to be more frequent in women with recurrent pregnancy loss. It is possible that disruption of VEGF function and placental angiogenesis can contribute to pregnancy loss in women with recurrent pregnancy loss.
血管内皮生长因子（VEGF）调节内皮细胞的增殖、迁移和分化。VEGF在胎盘形成过程中的血管生成中起关键作用。我们研究了VEGF基因多态性是否与复发性流产相关。2010年至2011年期间，在土耳其布尔萨地区招募了38例复发性流产妇女和30例有活产子女的对照妇女。通过对从白细胞获得的DNA样本进行PCR-RFLP分析来评估VEGF基因多态性。使用适当的引物研究DNA片段。分别使用MnII、BgIII、BshI2361、Hsp92II限制酶对1154 G/A、2578 C/A、460 C/T和936 C/T多态性进行SNP扫描。2578 C/A、460 C/T、936 C/T多态性的频率在对照组和复发性流产妇女之间无显著差异。然而，1154 G/A多态性A/A基因型在复发性流产组中的患病率显著更高（23.7%对3.4%）。VEGF基因的四种常见多态性之一在复发性流产妇女中更为常见。VEGF功能和胎盘血管生成的破坏可能导致复发性流产妇女的流产。
