Barros M P S, Silveira-Filho V M, Lins R H F B, Oliveira M B M, Almeida A M P, Leal-Balbino T C
Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Recife, PE, Brasil.
Genet Mol Res. 2013 Apr 17;12(2):1294-302. doi: 10.4238/2013.January.4.23.
We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak strains, as identical pulsotype patterns were found among outbreak strains and strains obtained from surveillance. However, it was able to detect intraspecific genetic diversity among Brazilian strains. This PFGE technique was able to differentiate a homogeneous group of Brazilian Y. pestis strains.
我们通过脉冲场凝胶电泳（PFGE）对巴西鼠疫耶尔森菌菌株进行了亚型分析。我们使用了22株巴西鼠疫耶尔森菌菌株进行此项分析：17株来自一次疫情爆发，5株来自地方性常规监测。这些菌株被分为2组（I组和II组）、8个亚组（A - H组）以及19种PFGE图谱或脉冲型。PFGE未能区分疫情爆发菌株和非疫情爆发菌株，因为在疫情爆发菌株和监测获得的菌株中发现了相同的脉冲型模式。然而，它能够检测出巴西菌株中的种内遗传多样性。这种PFGE技术能够区分一组同质化的巴西鼠疫耶尔森菌菌株。
