Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal.
G3 (Bethesda). 2013 Jan;3(1):1-4. doi: 10.1534/g3.112.004481. Epub 2013 Jan 1.
Among all organisms, Drosophila melanogaster has the most extensive well-characterized collection of large-scale chromosome rearrangements. Compound chromosomes, rearrangements in which homologous chromosome arms share a centromere, have proven especially useful in genetic-based surveys of the entire genome. However, their potential has not been fully realized because compound autosome stocks are refractile to standard genetic manipulations: if outcrossed, they yield inviable aneuploid progeny. Here we describe two strategies, cold-shock and use of the bubR1 mutant alleles, to produce nullo gametes through nondisjunction. These gametes are complementary to the compound chromosome-bearing gametes and thus produce viable progeny. Using these techniques, we created a compound chromosome two C(2)EN stock bearing a red fluorescent protein-histone transgene, facilitating live analysis of these unusually long chromosomes.
在所有生物中，黑腹果蝇拥有最广泛、特征最明显的大规模染色体重排。同源染色体臂共享着丝粒的复合染色体，在全基因组的基于遗传的调查中特别有用。然而，它们的潜力尚未得到充分实现，因为复合常染色体品系对标准遗传操作具有抗性：如果杂交，它们会产生不能存活的非整倍体后代。在这里，我们描述了两种策略，冷休克和使用 bubR1 突变等位基因，通过不分离产生 null 配子。这些配子与携带复合染色体的配子互补，因此可以产生可育的后代。使用这些技术，我们创建了一个携带红色荧光蛋白-组蛋白转基因的复合染色体 2CEN 品系，便于对这些异常长的染色体进行活体分析。
