乳腺癌中整合素α7（ITGA7）、富含半胱氨酸的血管内皮细胞生长因子样蛋白1（SVEP1）、 tensin蛋白1（TNS1）、 latrophilin 3（LPHN3）、 Semaphorin 3G（SEMA3G）、 Klotho β（KLB）和基质金属蛋白酶13（MMP13）mRNA表达的定量实时逆转录聚合酶链反应
Quantitative real-time RT-PCR of ITGA7, SVEP1, TNS1, LPHN3, SEMA3G, KLB and MMP13 mRNA expression in breast cancer.
作者信息
Kotepui Manas, Thawornkuno Charin, Chavalitshewinkoon-Petmitr Porntip, Punyarit Phaibul, Petmitr Songsak
机构信息
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
出版信息
Asian Pac J Cancer Prev. 2012;13(11):5879-82. doi: 10.7314/apjcp.2012.13.11.5879.
Breast cancer is the leading cause of cancer deaths among women worldwide, including Thailand. In the present study, the differential mRNA expression of SVEP1, LPHN3, KLB, ITGA7, SEMA3G, TNS1 and MMP13 genes was examined in breast cancer using quantitative real-time reverse transcription polymerase chain reaction (QRT-PCR). Among these genes, increased LPHN3 and MMP13 mRNA expression levels correlated with axillary-node metastasis (P=0.02). Multiple logistic regression analysis revealed that LPHN3 and MMP13 mRNA expression is significantly associated with axillary node status in breast cancer (P=0.04).
乳腺癌是包括泰国在内的全球女性癌症死亡的主要原因。在本研究中，使用定量实时逆转录聚合酶链反应（QRT-PCR）检测了乳腺癌中SVEP1、LPHN3、KLB、ITGA7、SEMA3G、TNS1和MMP13基因的差异mRNA表达。在这些基因中，LPHN3和MMP13 mRNA表达水平升高与腋窝淋巴结转移相关（P=0.02）。多因素逻辑回归分析显示，LPHN3和MMP13 mRNA表达与乳腺癌腋窝淋巴结状态显著相关（P=0.04）。
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