miR-152/TNS1 轴通过 Akt/mTOR/RhoA 通路抑制非小细胞肺癌进展。

miR-152/TNS1 axis inhibits non-small cell lung cancer progression through Akt/mTOR/RhoA pathway.

机构信息

Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China.

Department of Pathology, Xi'an Central Hospital, Xi'an, Shaanxi 7100033, P.R. China.

出版信息

Biosci Rep. 2021 Jan 29;41(1). doi: 10.1042/BSR20201539.

AIM

The purpose of the present study was to explore the function and mechanism of tensin 1 (TNS1) in non-small cell lung cancer (NSCLC) progression.

METHODS

The expression of TNS1 in NSCLC cells and tissues was assessed by RT-PCR and Western blot. Besides, Kaplan-Meier survival analysis was recruited to explore the association between TNS1 and NSCLC. Cell growth was analyzed by MTT and flow cytometry assay, while cell metastasis was determined by wound healing and transwell assays. The targeting relationship between TNS1 and miR-152 was assessed by luciferase activity assays. And Western blot was employed to determine the expression of related proteins of Akt/mTOR/RhoA pathway.

RESULTS

TNS1 level was boosted in NSCLC cells and tissues, related to the prognosis of NSCLC patients. Furthermore, it was proved that TNS1 promoted the growth and metastasis of NSCLC cells via Akt/mTOR/RhoA pathway. And miR-152 targeted TNS1 to affect the progression of NSCLC.

CONCLUSION

miR-152/TNS1 axis inhibits the progression of NSCLC by Akt/mTOR/RhoA pathway.

目的

本研究旨在探讨张力蛋白 1（TNS1）在非小细胞肺癌（NSCLC）进展中的作用和机制。

方法

采用 RT-PCR 和 Western blot 检测 NSCLC 细胞和组织中 TNS1 的表达。此外，还进行了 Kaplan-Meier 生存分析，以探讨 TNS1 与 NSCLC 之间的关系。通过 MTT 和流式细胞术分析细胞生长，通过划痕愈合和 Transwell 测定分析细胞迁移。通过荧光素酶活性测定评估 TNS1 与 miR-152 之间的靶向关系。并用 Western blot 检测 Akt/mTOR/RhoA 通路相关蛋白的表达。