Mueller C R, Maire P, Schibler U
Department of Molecular Biology, University of Geneva, Switzerland.
Cell. 1990 Apr 20;61(2):279-91. doi: 10.1016/0092-8674(90)90808-r.
The full-length cDNA for a transcriptional activator, DBP, that binds to the D site of the albumin promoter has been cloned. DBP belongs to a family of related transcription factors including Fos, Jun, CREB, and C/EBP, which share a conserved basic domain. However, unlike most other members of this family, DBP does not contain a "leucine zipper" structure. Among several rat tissues tested, significant levels of its protein are only observed in liver; yet, with the exception of testis, DBP mRNA is present in all of the examined tissues. DBP as well as its mRNA accumulate to significant levels only in adult animals. During chemically induced liver regeneration, DBP expression is rapidly down-regulated, suggesting that DBP may be involved in the proliferation control of hepatocytes. This cell growth-dependent expression of DBP, in contrast to its tissue specificity, appears to be controlled at the level of mRNA accumulation.
已克隆出一种与白蛋白启动子D位点结合的转录激活因子DBP的全长互补DNA。DBP属于一个相关转录因子家族，包括Fos、Jun、CREB和C/EBP，它们共享一个保守的碱性结构域。然而，与该家族的大多数其他成员不同，DBP不包含“亮氨酸拉链”结构。在测试的几种大鼠组织中，仅在肝脏中观察到其蛋白质的显著水平；然而，除睾丸外，DBP mRNA存在于所有检测的组织中。DBP及其mRNA仅在成年动物中积累到显著水平。在化学诱导的肝脏再生过程中, DBP表达迅速下调，这表明DBP可能参与肝细胞的增殖控制。与组织特异性相反，DBP的这种细胞生长依赖性表达似乎在mRNA积累水平上受到控制。
