一种在非洲爪蟾中与TFIIIA结构相似、仅与5S RNA结合的指状蛋白。

A finger protein structurally similar to TFIIIA that binds exclusively to 5S RNA in Xenopus.

作者信息

Joho K E, Darby M K, Crawford E T, Brown D D

机构信息

Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210.

出版信息

Cell. 1990 Apr 20;61(2):293-300. doi: 10.1016/0092-8674(90)90809-s.

DOI:10.1016/0092-8674(90)90809-s

PMID:2331751

Abstract

A 5S RNA binding protein (p43) in Xenopus is a major constituent of oocytes and comprises part of a 42S ribonucleoprotein storage particle. We have cloned and sequenced p43 cDNA from X. laevis and X. borealis as well as the cDNA for X. borealis TFIIIA. Like TFIIIA, p43 has nine zinc fingers, seven of which are exactly the same size as their counterparts in TFIIIA. Amino acid homology between the two proteins is restricted mainly to conserved residues characteristic of zinc fingers. In contrast to TFIIIA, which binds specifically to both 5S RNA and 5S RNA genes, p43 binds exclusively to 5S RNA.

摘要

非洲爪蟾中的一种5S RNA结合蛋白（p43）是卵母细胞的主要成分，并且是42S核糖核蛋白储存颗粒的一部分。我们已经从非洲爪蟾和北方爪蟾中克隆并测序了p43 cDNA以及北方爪蟾TFIIIA的cDNA。与TFIIIA一样，p43有九个锌指结构，其中七个与TFIIIA中的对应锌指结构大小完全相同。这两种蛋白质之间的氨基酸同源性主要限于锌指结构的保守残基。与能特异性结合5S RNA和5S RNA基因的TFIIIA不同，p43仅与5S RNA结合。