Mack Sarah A, Maltby Kathleen M, Hilton Matthew J
University of Rochester Medical Center, Rochester, NY, USA.
Methods Mol Biol. 2014;1130:165-183. doi: 10.1007/978-1-62703-989-5_12.
Cartilage and bone are specialized skeletal tissues composed of unique extracellular matrices. Bone, in particular, has a highly calcified or mineralized matrix that makes microtomy and standard histological studies very challenging. Therefore, methods to appropriately fix and decalcify mineralized skeletal tissues have been developed to allow for paraffin processing and standard microtomy. In this chapter, we illustrate methods for tissue grossing, fixation, decalcification, paraffin processing, embedding, sectioning, and routine histological staining of demineralized murine skeletal tissues. We also discuss methods for decalcified frozen sectioning of skeletal tissues with and without the use of a tape-transfer system.
软骨和骨是由独特的细胞外基质组成的特殊骨骼组织。特别是骨，其具有高度钙化或矿化的基质，这使得切片和标准组织学研究极具挑战性。因此，已开发出适当固定和脱钙矿化骨骼组织的方法，以实现石蜡包埋处理和标准切片。在本章中，我们阐述了脱钙小鼠骨骼组织的大体标本制作、固定、脱钙、石蜡包埋处理、包埋、切片及常规组织学染色的方法。我们还讨论了使用和不使用胶带转移系统对骨骼组织进行脱钙冰冻切片的方法。
