Torres-Aleman I, Naftolin F, Robbins R J
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
Brain Res Dev Brain Res. 1990 Mar 1;52(1-2):253-7. doi: 10.1016/0165-3806(90)90242-q.
The existence of different growth factors within a single brain region suggests that developing brain cells are exposed to a variety of trophic factors throughout neurogenesis. Cooperative interactions between growth factors are known to orchestrate growth and differentiation of various cell types. We explored the possibility that two growth factors may interact in promoting in vitro growth in fetal hypothalamic cells. We found that basic fibroblast growth factor (b-FGF) exerts trophic effects on primary mixed hypothalamic cell cultures, on enriched hypothalamic neuronal cultures, and on hypothalamic glial cultures. In addition, b-FGF increased the growth rate of two virally transformed hypothalamic cell lines. Since insulin-like growth factor I (IGF-I) also promotes growth of rat hypothalamic cells in vitro, we examined the combined effects of b-FGF and IGF-I on hypothalamic cells. Significantly higher numbers of neurite-bearing cells were present in primary mixed hypothalamic cultures when b-FGF and IGF-I were added together than were added separately. The effect was additive. These results establish b-FGF as a putative hypothalamic neurotrophic factor and demonstrate potential coordinate interactions between IGF-I and b-FGF in stimulating the growth or survival of developing hypothalamic cells.
在单一脑区内存在不同的生长因子，这表明发育中的脑细胞在整个神经发生过程中会接触到多种营养因子。已知生长因子之间的协同相互作用可协调各种细胞类型的生长和分化。我们探讨了两种生长因子在促进胎儿下丘脑细胞体外生长过程中可能相互作用的可能性。我们发现碱性成纤维细胞生长因子（b-FGF）对原代混合下丘脑细胞培养物、富集的下丘脑神经元培养物以及下丘脑神经胶质培养物均具有营养作用。此外，b-FGF提高了两种病毒转化的下丘脑细胞系的生长速率。由于胰岛素样生长因子I（IGF-I）在体外也能促进大鼠下丘脑细胞的生长，我们研究了b-FGF和IGF-I对下丘脑细胞的联合作用。当b-FGF和IGF-I一起添加到原代混合下丘脑培养物中时，出现的有神经突细胞的数量显著高于单独添加时。这种效应是相加的。这些结果确立了b-FGF作为一种假定的下丘脑神经营养因子，并证明了IGF-I和b-FGF在刺激发育中的下丘脑细胞生长或存活方面可能存在协同相互作用。
