人胆汁中结合胆汁酸的同步测定

Simultaneous determination of conjugated bile acids in human bile.

作者信息

Pang C P, Mok S D, Lam P K, Varma J, Li A K

机构信息

Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin.

出版信息

Clin Biochem. 1990 Feb;23(1):85-90. doi: 10.1016/0009-9120(90)90543-4.

DOI:10.1016/0009-9120(90)90543-4

PMID:2331806

Abstract

We describe an optimized liquid chromatographic method for simultaneous analysis of 10 conjugated bile acids in gall bladder and ductal bile. A quick and effective one-step purification with Sep-pak C-18 was adopted. We used a reverse phase C18 stainless steel column and an isocratic mobile phase in a flow programme and monitored the column effluent at 205 nm. The within-day CV ranged from 0.3 to 1.8%, and the between-day CV from 1.2 to 7.2%. Absolute analytical recovery ranged from 89 to 107%. Linearity ranged from 0.3 to 3 mg/mL for most bile acids. The chromatographic analysis was completed in 18 min.

摘要

我们描述了一种用于同时分析胆囊和胆管胆汁中10种结合胆汁酸的优化液相色谱方法。采用Sep-pak C-18进行快速有效的一步纯化。我们使用反相C18不锈钢柱和等度流动相进行梯度洗脱，并在205 nm波长处监测柱流出物。日内变异系数（CV）范围为0.3%至1.8%，日间CV范围为1.2%至7.2%。绝对分析回收率范围为89%至107%。大多数胆汁酸的线性范围为0.3至3 mg/mL。色谱分析在18分钟内完成。