Goto J, Hasegawa M, Kato H, Nambara T
Clin Chim Acta. 1978 Jul 1;87(1):141-7. doi: 10.1016/0009-8981(78)90068-2.
A method for simultaneous determination of major bile acids in human bile without prior hydrolysis is described. The unconjugated, glycine- and taurine-conjugated bile acids are separated into groups by ion-exchange chromatography on a newly developed lipophilic gel, piperidinohydroxypropyl Sephadex LH-20 (PHP-LH-20). Subsequently, resolution of each group into cholate, ursodeoxycholate, chenodeoxycholate, deoxycholate and lithocholate is attained into two stages by high-performance liquid chromatography on a micron-Bondapak C18 column. First, 0.3% ammonium carbonate/acetonitrile (9 : 4, v/v) is used for separation of the latter three as a mobile phase. Cholate and ursodeoxycholate are separated in 0.3% ammonium carbonate/acetonitrile (11 : 4, v/v). The present method is applicable to quantitation of free and conjugated bile acids in human bile with satisfactory accuracy and precision.
本文描述了一种无需预先水解即可同时测定人胆汁中主要胆汁酸的方法。未结合的、甘氨酸和牛磺酸结合的胆汁酸通过在新开发的亲脂性凝胶哌啶羟丙基葡聚糖LH - 20（PHP - LH - 20）上进行离子交换色谱法分离成不同组。随后，通过在微米级Bondapak C18柱上进行高效液相色谱法，分两个阶段将每组中的胆酸盐、熊去氧胆酸盐、鹅去氧胆酸盐、脱氧胆酸盐和石胆酸盐分离。首先，使用0.3%碳酸铵/乙腈（9 : 4，v/v）作为流动相分离后三种胆汁酸。胆酸盐和熊去氧胆酸盐在0.3%碳酸铵/乙腈（11 : 4，v/v）中分离。本方法适用于定量人胆汁中游离和结合胆汁酸，具有令人满意的准确度和精密度。
