Department of Zoology, Tinbergen Building, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
Development. 2013 Feb;140(4):730-9. doi: 10.1242/dev.082982. Epub 2013 Jan 14.
Recent advances in a number of systems suggest many genes involved in orchestrating regeneration are redeployed from similar processes in development, with others being novel to the regeneration process in particular lineages. Of particular importance will be understanding the architecture of regenerative genetic regulatory networks and whether they are conserved across broad phylogenetic distances. Here, we describe the role of the conserved TALE class protein PBX/Extradenticle in planarians, a representative member of the Lophotrocozoa. PBX/Extradenticle proteins play central roles in both embryonic and post-embryonic developmental patterning in both vertebrates and insects, and we demonstrate a broad requirement during planarian regeneration. We observe that Smed-pbx has pleiotropic functions during regeneration, with a primary role in patterning the anterior-posterior (AP) axis and AP polarity. Smed-pbx is required for expression of polarity determinants notum and wnt1 and for correct patterning of the structures polarized along the AP axis, such as the brain, pharynx and gut. Overall, our data suggest that Smed-pbx functions as a central integrator of positional information to drive patterning of regeneration along the body axis.
许多系统的最新进展表明，许多参与协调再生的基因是从发育过程中的类似过程中重新利用的，而其他基因则是特定谱系再生过程中特有的。特别重要的是要了解再生遗传调控网络的结构，以及它们是否在广泛的系统发育距离上保持保守。在这里，我们描述了保守的 TALE 类蛋白 PBX/Extradenticle 在扁形动物中的作用，扁形动物是 Lophotrocozoa 的一个代表成员。PBX/Extradenticle 蛋白在脊椎动物和昆虫的胚胎和胚胎后发育模式中都发挥着核心作用，我们在扁形动物再生过程中证明了其广泛的需求。我们观察到 Smed-pbx 在再生过程中具有多种功能，主要作用是对前后（AP）轴和 AP 极性进行模式化。Smed-pbx 对于极性决定因素 notum 和 wnt1 的表达以及沿着 AP 轴极化的结构的正确模式化是必需的，例如大脑、咽和肠道。总的来说，我们的数据表明，Smed-pbx 作为位置信息的中央整合因子，驱动沿身体轴的再生模式形成。
