Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Development. 2013 Feb;140(4):820-30. doi: 10.1242/dev.083725. Epub 2013 Jan 14.
Neural crest (NC) cells emerge from the dorsal trunk neural tube (NT) and migrate ventrally to colonize neuronal derivatives, as well as dorsolaterally to form melanocytes. Here, we test whether different dorsoventral levels in the NT have similar or differential ability to contribute to NC cells and their derivatives. To this end, we precisely labeled NT precursors at specific dorsoventral levels of the chick NT using fluorescent dyes and a photoconvertible fluorescent protein. NT and NC cell dynamics were then examined in vivo and in slice culture using two-photon and confocal time-lapse imaging. The results show that NC precursors undergo dynamic rearrangements within the neuroepithelium, yielding an overall ventral to dorsal movement toward the midline of the NT, where they exit in a stochastic manner to populate multiple derivatives. No differences were noted in the ability of precursors from different dorsoventral levels of the NT to contribute to NC derivatives, with the exception of sympathetic ganglia, which appeared to be 'filled' by the first population to emigrate. Rather than restricted developmental potential, however, this is probably due to a matter of timing.
神经嵴（NC）细胞起源于背侧神经管（NT）的主干，并向腹侧迁移以殖民神经元衍生物，以及向背外侧迁移以形成黑色素细胞。在这里，我们测试 NT 中的不同背腹水平是否具有相似或不同的能力来产生 NC 细胞及其衍生物。为此，我们使用荧光染料和光可转换荧光蛋白，精确标记鸡 NT 特定背腹水平的 NT 前体。然后使用双光子和共聚焦延时成像在体内和切片培养中检查 NT 和 NC 细胞的动力学。结果表明，NC 前体在神经上皮内经历动态重排，导致总体上向 NT 的中线腹侧到背侧移动，它们以随机的方式从中逸出，以填充多个衍生物。来自 NT 不同背腹水平的前体产生 NC 衍生物的能力没有差异，除了交感神经节，它似乎被第一批迁移的细胞“填充”。然而，这不是由于发育潜力受限，而是可能由于时间因素。
