H. Jones: Research Institute for Sport and Exercise Sciences, Tom Reilly Building, Liverpool John Moores University, Byrom Street Campus, Liverpool L3 3AF, UK.
J Physiol. 2013 Mar 15;591(6):1475-87. doi: 10.1113/jphysiol.2012.246918. Epub 2013 Jan 14.
Polycystic ovary syndrome (PCOS) is associated with cardiovascular disease. The contribution of the nitric oxide (NO) dilator system to cutaneous endothelial dysfunction is currently unknown in PCOS. Our aim was to examine whether women with PCOS demonstrate impaired cutaneous microvascular NO function and whether exercise training can ameliorate any impairment. Eleven women with PCOS (age, 29 ± 7 years; body mass index, 34 ± 6 kg m(-2)) were compared with six healthy obese control women (age, 29 ± 7 years; body mass index, 34 ± 5 kg m(-2)). Six women with PCOS (30 ± 7 years; 31 ± 6 kg m(-2)) then completed 16 weeks of exercise training. Laser Doppler flowmetry, combined with intradermal microdialysis of l-N(G)-monomethyl-l-arginine, a nitric oxide antagonist, in response to incremental local heating of the forearm was assessed in women with PCOS and control women, and again in women with PCOS following exercise training. Cardiorespiratory fitness, homeostasis model assessment for insulin resistance, hormone and lipid profiles were also assessed. Differences between women with PCOS and control women and changes with exercise were analysed using Student's unpaired t tests. Differences in the contribution of NO to cutaneous blood flow [expressed as a percentage of maximal cutaneous vasodilatation (CVCmax)] were analysed using general linear models. At 42°C heating, cutaneous NO-mediated vasodilatation was attenuated by 17.5%CVCmax (95% confidence interval, 33.3, 1.7; P = 0.03) in women with PCOS vs. control women. Exercise training improved cardiorespiratory fitness by 5.0 ml kg(-1) min(-1) (95% confidence interval, 0.9, 9.2; P = 0.03) and NO-mediated cutaneous vasodilatation at 42°C heating by 19.6% CVCmax (95% confidence interval, 4.3, 34.9; P = 0.02). Cutaneous microvascular NO function is impaired in women with PCOS compared with obese matched control women but can be improved with exercise training.
多囊卵巢综合征（PCOS）与心血管疾病有关。目前尚不清楚一氧化氮（NO）舒张系统对多囊卵巢综合征患者皮肤内皮功能障碍的贡献。我们的目的是研究多囊卵巢综合征患者是否存在皮肤微血管一氧化氮功能障碍，以及运动训练是否可以改善这种障碍。
将 11 名多囊卵巢综合征患者（年龄 29 ± 7 岁；体重指数 34 ± 6 kg/m²）与 6 名健康肥胖对照组女性（年龄 29 ± 7 岁；体重指数 34 ± 5 kg/m²）进行比较。然后，6 名多囊卵巢综合征患者（30 ± 7 岁；31 ± 6 kg/m²）完成了 16 周的运动训练。使用激光多普勒血流仪，结合前臂局部加热时皮内微透析 l-N（G）-单甲基-l-精氨酸（一种一氧化氮拮抗剂），评估多囊卵巢综合征患者和对照组女性的皮肤血流，以及运动训练后多囊卵巢综合征患者的皮肤血流。还评估了心肺适应性、胰岛素抵抗的稳态模型评估、激素和血脂谱。使用学生非配对 t 检验分析多囊卵巢综合征患者与对照组女性之间的差异以及运动训练后的变化。使用一般线性模型分析 NO 对皮肤血流的贡献差异（表示为最大皮肤血管扩张率的百分比）。
在 42°C 加热时，多囊卵巢综合征患者皮肤的一氧化氮介导的血管扩张作用降低了 17.5% CVCmax（95%置信区间，33.3，1.7；P = 0.03）。与对照组女性相比，运动训练使心肺适应性提高了 5.0 ml/kg/min（95%置信区间，0.9，9.2；P = 0.03），42°C 加热时的一氧化氮介导的皮肤血管扩张作用提高了 19.6% CVCmax（95%置信区间，4.3，34.9；P = 0.02）。与肥胖匹配的对照组女性相比，多囊卵巢综合征患者的皮肤微血管一氧化氮功能受损，但运动训练可以改善。
