Kearney J N, Wheldon L A, Gowland G
Yorkshire Regional Tissue Bank, Pinderfields General Hospital, Wakefield, United Kingdom.
Cryobiology. 1990 Apr;27(2):164-70. doi: 10.1016/0011-2240(90)90008-r.
Skin from an inbred strain of hairless mouse was used as a homogeneous model tissue for studies of skin cryopreservation. Tetrazolium reductase enzyme activity was used to assess tissue viability. Hepes-buffered 199 tissue culture medium was confirmed to be a suitable basal medium, to which cryoprotectants were added. Addition of serum to the cryoprotective cocktail had no beneficial effect. Three cryoprotectants, dimethyl sulfoxide, ethanediol, and glycerol were evaluated. There was no evidence of specific toxicity attributable to the cryoprotective agents during the permeation period; however, short permeation times at low temperature were associated with maximum skin viability. Following freezing and thawing, higher viabilities were obtained when using a slow (-1 degree C min-1) or medium (-60 degree C min-1) rather than a fast (immersion in liquid nitrogen) cooling rate. Dimethyl sulfoxide was a marginally better cryoprotectant overall, although this difference was not statistically significant.
将近交系无毛小鼠的皮肤用作皮肤冷冻保存研究的均质模型组织。用四氮唑还原酶活性评估组织活力。已证实Hepes缓冲的199组织培养基是合适的基础培养基，并向其中添加了冷冻保护剂。向冷冻保护剂混合物中添加血清没有有益效果。评估了三种冷冻保护剂，即二甲亚砜、乙二醇和甘油。在渗透期没有证据表明冷冻保护剂具有特定毒性；然而，低温下较短的渗透时间与最大皮肤活力相关。冷冻和解冻后，使用缓慢（-1℃/分钟）或中等（-60℃/分钟）而非快速（浸入液氮）冷却速率可获得更高的活力。总体而言，二甲亚砜是一种稍好的冷冻保护剂，尽管这种差异没有统计学意义。
