Verbeken Gilbert, Verween Gunther, De Vos Daniel, Pascual Bruno, De Corte Peter, Richters Cornelia, De Coninck Arlette, Roseeuw Diane, Ectors Nadine, Rose Thomas, Jennes Serge, Pirnay Jean-Paul
Skin- and Keratinocyte Bank, Laboratory for Molecular and Cellular Technology, Burn Wound Centre, Queen Astrid Military Hospital, Brussels, Belgium.
Cell Tissue Bank. 2012 Mar;13(1):1-7. doi: 10.1007/s10561-011-9244-6. Epub 2011 Mar 1.
Human donor skin allografts are suitable and much used temporary biological (burn) wound dressings. They prepare the excised wound bed for final autografting and form an excellent substrate for revascularisation and for the formation of granulation tissue. Two preservation methods, glycerol preservation and cryopreservation, are commonly used by tissue banks for the long-term storage of skin grafts. The burn surgeons of the Queen Astrid Military Hospital preferentially use partly viable cryopreserved skin allografts. After mandatory 14-day bacterial and mycological culture, however, approximately 15% of the cryopreserved skin allografts cannot be released from quarantine because of positive culture. To maximize the use of our scarce and precious donor skin, we developed a glycerolisation-based recovery method for these culture positive cryopreserved allografts. The inactivation and preservation method, described in this paper, allowed for an efficient inactivation of the colonising bacteria and fungi, with the exception of spore-formers, and did not influence the structural and functional aspects of the skin allografts.
人供体皮肤异体移植物是合适且常用的临时性生物（烧伤）伤口敷料。它们为最终的自体移植准备切除后的伤口床，并为血管再生和肉芽组织形成提供优良基质。组织库通常使用两种保存方法，即甘油保存和冷冻保存，用于皮肤移植物的长期储存。阿斯特丽德女王军事医院的烧伤外科医生优先使用部分存活的冷冻保存皮肤异体移植物。然而，在进行强制性的14天细菌和真菌培养后，约15%的冷冻保存皮肤异体移植物因培养呈阳性而无法解除检疫。为了最大限度地利用我们稀缺而珍贵的供体皮肤，我们针对这些培养呈阳性的冷冻保存异体移植物开发了一种基于甘油化的恢复方法。本文所述的灭活和保存方法能够有效灭活定植细菌和真菌（芽孢形成菌除外），且不影响皮肤异体移植物的结构和功能。
