IDF Medical Corps, Ramat Gan, Israel.
PLoS One. 2013;8(1):e49852. doi: 10.1371/journal.pone.0049852. Epub 2013 Jan 8.
Trauma is a leading cause of death among young individuals globally and uncontrolled hemorrhage is the leading cause of preventable death. Controlling hemorrhage from a solid organ is often very challenging in military as well as civilian setting. Recent studies demonstrated reversible vasoconstriction and irreversible thrombosis following application of microseconds-long electrical pulses. The current paper describes for the first time reduction in bleeding from the injured liver in rat and rabbit model in-vivo. We applied short (25 and 50 µs) electrical pulses of 1250 V/cm to rats and rabbit liver following induction of standardized penetrating injury and measured the amount of bleeding into the abdominal cavity one hour post injury. We found a 60 and 36 percent reduction in blood volume in rats treated by 25 µs and 50 µs, respectively (P<0.001). Similar results were found for the rabbit model. Finite element simulation revealed that the effect was likely non-thermal. Histological evaluation found local cellular injury with intravascular thrombosis. Further research should be done to fully explore the mechanism of action and the potential use of short electric pulses for hemorrhage control.
创伤是全球年轻人死亡的主要原因，而无法控制的出血是可预防死亡的主要原因。在军事和民用环境中，控制实质性器官的出血通常极具挑战性。最近的研究表明，在应用微秒长的电脉冲后，会出现可逆的血管收缩和不可逆的血栓形成。本文首次描述了在大鼠和兔模型中，电脉冲可减少受伤肝脏的出血。我们在诱导标准穿透性损伤后，对大鼠和兔肝脏施加短（25 和 50µs）、1250V/cm 的电脉冲，并测量损伤后 1 小时腹腔内的出血量。结果发现，25µs 和 50µs 处理组的大鼠出血量分别减少了 60%和 36%（P<0.001）。兔模型也得到了类似的结果。有限元模拟表明，这种效果可能是非热的。组织学评估发现局部细胞损伤伴有血管内血栓形成。应进一步研究以充分探索短电脉冲在控制出血方面的作用机制和潜在用途。
