Precup C G, Gonganau-Nitu D, Scurtu R R, Dindelegan G, Biro A, Soritau O, Crişan C, Serban O, Pufu G, Ciuce C
Department of Surgery no 1, University of Medicine and Pharmacy "Iuliu Haţieganu", Cluj-Napoca, Romania.
Chirurgia (Bucur). 2010 Jan-Feb;105(1):71-6.
Colorectal cancer became one of the most frequent malignant conditions of the past two decades. Non-resecable liver metastases might be destroyed in situ by radiofrequency although the local recurrence is still very important. Laser-Doppler flowmetry has proved to be a simple technique for monitoring the microcirculation, hereby the tissue perfusion at the edge of the post radiofrequency necrosis. The aim of the study was to evaluate microcirculation using lasser-Doppler for hepatic tissue and peripheral tumour perfusion after radiofrequency and the influence of local temperature increasing at 42 degrees C on tissular perfusion. Colorectal adenocarcinoma (CC531s) was used for liver tumour inoculationin on 15 Wag/Rij rats. Twenty-one days after inoculation, perfusion in hepatic tissue, on the tumour before and after radiofrequency treatment was mesured. When hepatic tissue was heated at 42 degrees C there was an increase in tissular perfusion, on the other part, heating the tumoural tissue do not increase perfusion. After radiofrequency in the periphery of necrosis the perfusion was still present, despite a clear drop towards initial level. Assessing the local microcirculation and tissue temperature during RF ablation by Laser-Doppler might be useful not only for RF efficiency evaluation but also as an indication for associating adjuvant local chemotherapy.
在过去二十年中，结直肠癌已成为最常见的恶性疾病之一。尽管局部复发仍然非常重要，但不可切除的肝转移瘤可通过射频原位破坏。激光多普勒血流仪已被证明是一种监测微循环的简单技术，从而可监测射频坏死边缘的组织灌注。本研究的目的是使用激光多普勒评估射频后肝组织和外周肿瘤灌注的微循环，以及42℃局部升温对组织灌注的影响。将结直肠腺癌（CC531s）接种于15只Wag/Rij大鼠的肝脏以形成肿瘤。接种21天后，测量肝组织、射频治疗前后肿瘤的灌注情况。当肝组织加热至42℃时，组织灌注增加，而加热肿瘤组织则不会增加灌注。射频后坏死周边仍存在灌注，尽管明显降至初始水平。通过激光多普勒评估射频消融期间的局部微循环和组织温度不仅可能有助于评估射频效率，还可作为辅助局部化疗的指征。
