[不同染色质制备方法对大鼠肝脏亚核组分中锰离子依赖性多聚腺苷酸聚合酶活性分布的影响]
[Effect of various methods of preparation of chromatin on the distribution of Mn2+-dependent poly-A-polymerase activity in subnuclear fractions of rat liver].
作者信息
Corti A, Drusiani F, Rossini G P, Casti A
出版信息
Ateneo Parmense Acta Biomed. 1979;50(1):83-6.
PMID:233203
Abstract
The distribution of Mn2+-dependent poly(A) polymerase activity between chromatin and nucleoplasmic fractions in rat liver nuclei strongly depends on the method followed for the chromatin preparation. Currently used methods containing high salt cause a loss in bound activity not accompanied by a correspondent increase in the "free" one.
摘要
大鼠肝细胞核中，染色质和核质部分之间依赖锰离子的聚腺苷酸聚合酶活性分布，很大程度上取决于染色质制备所采用的方法。目前使用的含高盐的方法会导致结合活性丧失，且“游离”活性并未相应增加。
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