School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science & Technology (UNIST), 100 Banyeon-ri, Eonyang-eup, Ulju-gun, Ulsan 689-798, Republic of Korea.
Anal Chem. 2013 Mar 5;85(5):2954-60. doi: 10.1021/ac3036734. Epub 2013 Feb 13.
In this study, we describe a novel platform based on centrifugal microfluidics for simultaneous determination of nitrite, nitrate and nitrite, ammonium, orthophosphate, and silicate in water samples. All processes from sample metering to detection were integrated and automatically conducted on a rotating disc-shaped device. Fluid transfer was controlled by laser irradiation on the ferrowax-based microvalves. Liquid samples and reagents were pumped by centrifugal force in the rotating disc, and their positions and movements were controlled through a programmable light from a laser diode. This novel water analysis platform required only 500 μL of sample (100 μL for each nutrient) and 10-30 μL of reagents for colorimetric detection. In addition, the fully automated parallel processes and efficient mixing in the rotating disc allowed for a significant reduction in total analysis time (∼7 min 40 s) and increased accuracy. Validation with a seawater certified reference material indicated that the platform accurately measured nutrient concentrations in water samples. In addition, we showed that the nutrients in the seawater collected from Chunsu Bay in Korea measured by the proposed lab-on-a-disc and by a commercialized autoanalyzer are comparable.
在这项研究中，我们描述了一种基于离心微流控的新平台，用于同时测定水样中的亚硝酸盐、硝酸盐、亚硝酸盐、铵、正磷酸盐和硅酸盐。从样品计量到检测的所有过程都在一个旋转圆盘装置上集成并自动进行。流体转移由基于费罗蜡的微阀上的激光照射控制。液体样品和试剂通过离心力在旋转盘中泵送，其位置和运动通过激光二极管的可编程光进行控制。这种新型水分析平台仅需 500 μL 样品（每种养分 100 μL）和 10-30 μL 试剂即可进行比色检测。此外，旋转盘中的全自动并行处理和高效混合允许显著缩短总分析时间（约 7 分 40 秒）并提高准确性。使用海水认证参考物质进行验证表明，该平台能够准确测量水样中的养分浓度。此外，我们还表明，由提议的片上实验室和商业化自动分析仪测量的韩国春遂湾采集的海水中的养分是可比的。
