Samsung Electronics Co. Ltd., 416, Maetan-3Dong, Yeongtong-Gu, Suwon-City, Gyeonggi-Do 443-746, Korea.
Lab Chip. 2011 Jan 7;11(1):70-8. doi: 10.1039/c0lc00205d. Epub 2010 Nov 1.
We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable "lab-on-a-disc" containing different kinds of freeze-dried reagents for the blood chemistry analysis as well as reagents required for the immunoassay. The concentrations of different kinds of analytes are reported within 22 min by simply inserting a disc to a portable device. Using the innovative laser irradiated ferrowax microvalves together with the centrifugal microfluidics, the total process of plasma separation, metering, mixing, incubation, washing, and detection is fully automated. The analyzer is equipped with an optical detection module to measure absorbances at 10 different wavelengths to accommodate the various kinds of reaction protocols. Compared to the conventional blood analysis done in clinical laboratories, it is advantageous for point-of-care applications because it requires a smaller amount of blood (350 μL vs. 3 mL), takes less time (22 min vs. several days), does not require specially trained operators or expensive instruments to run biochemical analysis and immunoassay separately.
我们报告了一种完全集成的设备，它可以在一个圆盘上同时进行多种生化分析和夹心型免疫分析。全血直接应用于含有不同种类冻干试剂的一次性“盘上实验室”，用于血液化学分析以及免疫分析所需的试剂。只需将圆盘插入便携式设备，即可在 22 分钟内报告各种分析物的浓度。通过使用创新的激光照射铁蜡微阀和离心微流控技术，整个血浆分离、计量、混合、孵育、洗涤和检测过程完全自动化。该分析仪配备了一个光学检测模块，可测量 10 个不同波长的吸光度，以适应各种反应方案。与临床实验室中进行的常规血液分析相比，它更有利于即时护理应用，因为它只需要较少的血量（350μL 对 3mL），耗时更短（22 分钟对几天），不需要专门的操作人员或昂贵的仪器来分别进行生化分析和免疫分析。
