College of Information and Communication Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do 440-746, Korea.
Sensors (Basel). 2019 Jul 20;19(14):3207. doi: 10.3390/s19143207.
Lab-on-a-disc (LOD) has emerged as a promising candidate for a point-of-care testing (POCT) device because it can effectively integrate complex fluid manipulation steps using multiple layers of polymeric substrates. However, it is still highly challenging to design and fabricate temperature measurement and heating system in non-contact with the surface of LOD, which is a prerequisite to successful realization of DNA amplification especially with a rotatable disc. This study presents a Lab-on-a-disc (LOD)-based automatic loop-mediated isothermal amplification (LAMP) system, where a thermochromic coating (<~420 µm) was used to distantly measure the chamber's temperature and a micro graphite film was integrated into the chamber to remotely absorb laser beam with super high efficiency. We used a deep learning network to more consistently analyze the product of LAMP than we could with the naked eye. Consequently, both temperature heating and measurement were carried out without a physical contact with the surface of LOD. The experimental results show that the proposed approach, which no previous work has attempted, was highly effective in realizing LAMP in LOD.
碟式实验室（LOD）已成为一种有前途的即时检测（POCT）设备候选，因为它可以使用多层聚合物基底有效地集成复杂的流体处理步骤。然而，在不与 LOD 表面接触的情况下设计和制造温度测量和加热系统仍然极具挑战性，这是成功实现 DNA 扩增的前提，尤其是在可旋转的圆盘上。本研究提出了一种基于碟式实验室（LOD）的自动环介导等温扩增（LAMP）系统，其中使用热敏涂层（<~420 µm）远距离测量腔室的温度，并将微石墨薄膜集成到腔室中，以超高效地远程吸收激光束。我们使用深度学习网络比肉眼更一致地分析 LAMP 的产物。因此，无需与 LOD 表面物理接触即可进行温度加热和测量。实验结果表明，这种前所未有的方法在 LOD 中实现 LAMP 非常有效。
