丝裂原对培养早期大鼠肠系膜淋巴结淋巴细胞和脾细胞中己糖激酶、乳酸脱氢酶、柠檬酸合酶和谷氨酰胺酶最大活性的影响。

Effect of mitogens on the maximum activities of hexokinase, lactate dehydrogenase, citrate synthase and glutaminase in rat mesenteric lymph node lymphocytes and splenocytes during the early period of culture.

Keast D, Newsholme E A

Department of Biochemistry, University of Oxford, England.

Int J Biochem. 1990;22(2):133-6. doi: 10.1016/0020-711x(90)90174-2.

Abstract

The activities of hexokinase, lactate dehydrogenase and citrate synthase were maintained in mesenteric lymph node lymphocytes during 4 hr of culture: the activity of glutaminase increased during this period of time. 2. In splenocytes, the activity of hexokinase decreased markedly during the 4 hr period, whereas those of lactate dehydrogenase and glutaminase remained constant, and that of citrate synthase increased dramatically. 3. In both mesenteric lymphocytes and splenocytes, addition of the T-cell mitogens, phytohaemagglutinin or concanavalin-A, to the culture medium caused decreases in the activities of both hexokinase and citrate synthase. 4. In contrast, these mitogens increased the activity of glutaminase in both cell types. 5. Addition of the B-cell mitogen, bacterial lipopolysaccharide, had little effect on hexokinase, lactate dehydrogenase or citrate synthase but increased markedly that of glutaminase in mesenteric lymph node lymphocytes. 6. In splenocytes this mitogen prevented much of the decrease in hexokinase activity, increased the activities of citrate synthase and glutaminase but had little effect on that of lactate dehydrogenase.

摘要

在培养4小时期间，肠系膜淋巴结淋巴细胞中的己糖激酶、乳酸脱氢酶和柠檬酸合酶的活性得以维持：在此时间段内谷氨酰胺酶的活性增加。2. 在脾细胞中，己糖激酶的活性在4小时期间显著下降，而乳酸脱氢酶和谷氨酰胺酶的活性保持不变，柠檬酸合酶的活性则急剧增加。3. 在肠系膜淋巴细胞和脾细胞中，向培养基中添加T细胞有丝分裂原、植物血凝素或伴刀豆球蛋白A，会导致己糖激酶和柠檬酸合酶的活性降低。4. 相反，这些有丝分裂原会增加两种细胞类型中谷氨酰胺酶的活性。5. 添加B细胞有丝分裂原细菌脂多糖，对己糖激酶、乳酸脱氢酶或柠檬酸合酶影响不大，但会显著增加肠系膜淋巴结淋巴细胞中谷氨酰胺酶的活性。6. 在脾细胞中，这种有丝分裂原可防止己糖激酶活性的大部分下降，增加柠檬酸合酶和谷氨酰胺酶的活性，但对乳酸脱氢酶的活性影响不大。