饮食对不同年龄的有毛和无毛小鼠皮肤中谷氨酰胺酶、柠檬酸合酶、己糖激酶、6-磷酸果糖激酶和乳酸脱氢酶最大活性的影响。

The effects of diet on the maximal activities of glutaminase, citrate synthase, hexokinase, 6-phosphofructokinase and lactate dehydrogenase in the skin of haired and hairless mice of various ages.

Nguyen T D, Keast D

Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.

Int J Biochem. 1991;23(3):365-9. doi: 10.1016/0020-711x(91)90120-c.

Abstract

The maximal activities of hexokinase (HK), 6-phosphofructokinase (PFK), lactate dehydrogenase (LDH), citrate synthase (CS) and glutaminase (GLU) which provide a quantitative indices of flux through several important pathways have been measured in the skin of haired Balb/c and hairless Balb/c (nu/nu) mice under normal and dietary stress. 2. The skin of old haired mice exhibited higher PFK and LDH activities with lower HK, CS and GLU activities. All activities of enzymes associated with energy metabolism in the skin of old hairless mice were higher than those in the skin of haired mice. 3. HK, LDH, CS and GLU activities were maintained at normal levels in the skin of haired mice when these mice were fed diets deficient in energy or protein components (HPLE, LPNE). These enzymes however were severely suppressed when mice were fed a diet deficient in both energy and protein components (LPLE). Recovery of activities of these enzymes to the control level was observed when mice were refed with the normal diet for a week.

摘要

已在正常和饮食应激条件下，测量了有毛Balb/c小鼠和无毛Balb/c（nu/nu）小鼠皮肤中己糖激酶（HK）、6-磷酸果糖激酶（PFK）、乳酸脱氢酶（LDH）、柠檬酸合酶（CS）和谷氨酰胺酶（GLU）的最大活性，这些酶活性提供了通过几个重要途径通量的定量指标。2. 老年有毛小鼠的皮肤表现出较高的PFK和LDH活性，同时HK、CS和GLU活性较低。老年无毛小鼠皮肤中与能量代谢相关的所有酶活性均高于有毛小鼠皮肤中的酶活性。3. 当给有毛小鼠喂食缺乏能量或蛋白质成分的饮食（HPLE、LPNE）时，其皮肤中的HK、LDH、CS和GLU活性维持在正常水平。然而，当给小鼠喂食缺乏能量和蛋白质成分的饮食（LPLE）时，这些酶受到严重抑制。当小鼠重新喂食正常饮食一周后，观察到这些酶的活性恢复到对照水平。