Lee Seung Woo, Kang Sang Bum, Lee Dong Soo, Lee Jung Uee
Hepatogastroenterology. 2013 Jun;60(124):932-7. doi: 10.5754/hge121214. Epub 2013 Jan 16.
BACKGROUND/AIMS: There is increasing evidence that the PI3K/Akt signal pathway plays a crucial role in tumorigenesis and tumor progression. Akt protein modulates the function of numerous substrates related to the regulation of cell proliferation, such as cyclin dependent kinase inhibitors, including p27 and Cks1. The aim of this study was to investigate the immunohistochemical expressions of Akt, p27 and Cks1 proteins according to clinicopathological parameters such as gender, lymph node metastases, tumor invasion depth, the TNM stage and Lauren classification in gastric adenocarcinoma.
Immunohistochemical staining with monoclonal Akt, p27 and Cks1 antibodies was performed on the paraffin embedded specimens of 43 gastric adenocarcinomas.
The expression of Akt was increased more in the adenocarcinomas that showed lymph node involvement (53.5%) than in the adenocarcinomas that did not (9.3%) (p=0.007). The expression of Cks1 was increased more in the adenocarcinomas that showed lymph node involvement (39.5%) than in the adenocarcinomas that did not (7%) (p<0.043).
Akt and Cks1 are associated with lymph node metastasis in gastric adenocarcinoma. We suggest that Akt and Cks1 proteins might be related with poor prognosis in gastric adenocarcinoma.
背景/目的：越来越多的证据表明，PI3K/Akt信号通路在肿瘤发生和肿瘤进展中起关键作用。Akt蛋白可调节众多与细胞增殖调控相关的底物的功能，如细胞周期蛋白依赖性激酶抑制剂，包括p27和Cks1。本研究的目的是根据性别、淋巴结转移、肿瘤浸润深度、TNM分期和Lauren分类等临床病理参数，研究胃腺癌中Akt、p27和Cks1蛋白的免疫组化表达情况。
用单克隆Akt、p27和Cks1抗体对43例胃腺癌石蜡包埋标本进行免疫组化染色。
有淋巴结转移的腺癌中Akt的表达增加（53.5%），高于无淋巴结转移的腺癌（9.3%）（p=0.007）。有淋巴结转移的腺癌中Cks1的表达增加（39.5%），高于无淋巴结转移的腺癌（7%）（p<0.043）。
Akt和Cks1与胃腺癌的淋巴结转移有关。我们认为Akt和Cks1蛋白可能与胃腺癌的预后不良有关。
